biotinylated polyclonal human activin riib antibody (R&D Systems)
Structured Review

Biotinylated Polyclonal Human Activin Riib Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+anti+activin+a+antibody/pmc12489275-49-11-29?v=R%26D+Systems
Average 93 stars, based on 10 article reviews
Images
1) Product Images from "Gel Electrophoretic Detection of Black Market ACE‐031"
Article Title: Gel Electrophoretic Detection of Black Market ACE‐031
Journal: Drug Testing and Analysis
doi: 10.1002/dta.3898
Figure Legend Snippet: Flow scheme for the detection of black market ACE‐031 in serum and urine. Key elements are the IP step employing a polyclonal anti‐ACVR2B antibody as well as immunoblotting with a monoclonal anti‐ACVR2B antibody after electrophoretic separation.
Techniques Used: Western Blot
Figure Legend Snippet: Results of the inter‐laboratory myostatin inhibitors' study. Of the eight blind serum samples, the one containing black market ACE‐031 (Sample B) was correctly identified. IP with polyclonal ACVR2B antibody coated magnetic beads. Immunoblot after SDS‐PAGE with clone 60408 anti‐ACVR2B antibody. The red arrow indicates the monomer, and the green indicates the dimer.
Techniques Used: Magnetic Beads, Western Blot, SDS Page
Figure Legend Snippet: Method verification for the detection of BM ACE‐031 in rat serum (A) and rat urine (B). SDS‐PAGE and Western blot with clone 60408 anti‐ACVR2B antibody after IP with a polyclonal anti‐ACVR2B antibody. ACE‐031 monomer (red arrow). ACE‐031 dimer (green arrow). The negative control (0 ng/mL) shows no interferences in the mass range of the monomer and dimer.
Techniques Used: SDS Page, Western Blot, Negative Control
Figure Legend Snippet: Analysis of five female and five male rat serum samples 24/48/168 h after administration of BM ACE‐031 (red arrow). IP with polyclonal anti‐ACVR2B antibody followed by SDS‐PAGE and immunoblot with biotinylated clone 60408 anti‐ACVR2B antibody and streptavidin‐POD. Note the strong difference in signal intensity between the black market (red) product and the Reblozyl (blue), which indicates that the BM product contains a much lower amount of immunoreactive ACVR2B. NQC (negative rat serum), PQC (Reblozyl/BM ACE‐031 positive rat serum). Lanes marked with a “+”,“+/−”, or “−” refer to samples in which BM ACE‐031 was “clearly,” “not clearly,” or “not” identified.
Techniques Used: SDS Page, Western Blot
Figure Legend Snippet: In vitro study of BM ACE‐031 using human and rat liver microsomes. SDS‐PAGE and Western blot with biotinylated clone 60408 anti‐ACVR2B antibody and streptavidin‐POD.
Techniques Used: In Vitro, SDS Page, Western Blot